Journal: Frontiers in Endocrinology

Article Title: Aconine attenuates osteoclast-mediated bone resorption and ferroptosis to improve osteoporosis via inhibiting NF-κB signaling

doi: 10.3389/fendo.2023.1234563

Figure Lengend Snippet: AC reverses the high bone turnover makers and remodels the osteoporotic pathophysiological microenvironment in OVX mice. Serum levels of bone formation markers of (A) bone-specific alkaline (BALP) and (B) N-terminal propeptide of type I collagen (PINP). (C) Serum β-CTX levels of the bone resorption marker. n = 6. IHC staining of (D) Runx2 and (E) Osterix in the lumbar vertebrae of each group. The red arrows represented the positive expression. (F) Representative of TRAP staining among groups. The black arrows represented the TRAP-positive cells. (G, H) Quantification of Runx2 + and Osterix + cells. n = 4. (I) The ratio of the number of osteoclasts to per unit bone surface (OC.N/BS). n = 6. Data presented as means ± s.d. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The cleaved-off of type I collagen (PINP) (Sangon Biotech, D721053) and bone-specific alkaline phosphatase (BALP) (Sangon Biotech, D721049) were performed for bone formation, while carboxy-terminal cross-linking telopeptide of type I collagen (β-CTX) (Sangon Biotech, D721187) was detected for bone resorption.

Techniques: Marker, Immunohistochemistry, Expressing, Staining

Journal: International Journal of Molecular Medicine

Article Title: Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro

doi: 10.3892/ijmm.2018.3431

Figure Lengend Snippet: Immunohistochemical staining. Stronger positive expression (brown-yellow color) of Col II and weaker expression of Col I was observed in the nHC group compared with that in the HC and pure collagen groups, indicating that the chondrcocyte phenotype and biological functions could be maintained effectively when cells were cultured on nHC film for a long time period. HC, hydroxyapatite/collagen; nHC, nano-HC; calcein-AM/PI, acetoxymethyl/propidium iodide; TGF-β, transforming growth factor-β; COL-I, collagen type I; COL-II, collagen type II.

Article Snippet: Anti-COLIA1 and -COL1A2 antibodies and the immunohistochemistry (IHC) kit were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Staining, Expressing, Cell Culture

Journal: International Journal of Molecular Medicine

Article Title: Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro

doi: 10.3892/ijmm.2018.3431

Figure Lengend Snippet: RT-qPCR analysis. Cartilage matrix relative gene expression was analyzed by RT-qPCR. The chondrocytes were seeded on the nHC, HC and pure collagen films and cultured for 6 days. Gene expression in cells relative to the control group was calculated by the 2 −ΔΔCq method using the β-actin gene as the internal control. The data are reported as the mean ± standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. the collagen-based groups; # P<0.05, ## P<0.01 and ### P<0.001 for comparisons between the collagen-based groups and the TGF-β group. HC, hydroxyapatite/collagen; nHC, nano-HC; calcein-AM/PI, acetoxymethyl/propidium iodide; TGF-β, transforming growth factor-β; COL-I, collagen type I; COL-II, collagen type II; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.

Article Snippet: Anti-COLIA1 and -COL1A2 antibodies and the immunohistochemistry (IHC) kit were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Quantitative RT-PCR, Gene Expression, Cell Culture, Control, Standard Deviation, Reverse Transcription, Real-time Polymerase Chain Reaction